Journal: Antiviral research

Article Title: Serial Real-time RT-PCR and serology measurements substantially improve Zika and Dengue virus infection classification in a co-circulation area.

doi: 10.1016/j.antiviral.2019.104638

Figure Lengend Snippet: Table of Demographics for patients in this analysis

Article Snippet: Serologies of the three arboviruses were measured using commercial Enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions: anti-ZIKV specific-IgM (ZIKV-sIgM) and IgG (ZIKV-sIgG) ELISA (EuroImmun®, Lübeck, Germany), anti-CHIKV specific-IgM (CHIKV-sIgM) and IgG (CHIKV-sIgG) ELISA (EuroImmun®, Lübeck, Germany), anti-DENV specific-IgG (Panbio Dengue IgG Capture ELISA - panbio diagnostics®, Republic of Korea) and anti-DENV specific-IgG (Panbio Dengue IgM Capture ELISA - panbio diagnostics®, Republic of Korea).

Techniques:

Journal: Antiviral research

Article Title: Serial Real-time RT-PCR and serology measurements substantially improve Zika and Dengue virus infection classification in a co-circulation area.

doi: 10.1016/j.antiviral.2019.104638

Figure Lengend Snippet: Spaghetti plots for ZIKV and DENV sIgG and sIgM among the three cohorts: ZIKV-sIgG (row 1), ZIKV-sIgM (row 2), DENV-sIgG (row 3) and DENV-sIgM (row 4) by study visit for each cohort: Definitive Dengue, Definitive Zika and Household. Each colored line represents the trajectory of one subject in the study. The horizontal black line represents the manufacturer-recommended cutoff for defining seropositivity.

Article Snippet: Serologies of the three arboviruses were measured using commercial Enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions: anti-ZIKV specific-IgM (ZIKV-sIgM) and IgG (ZIKV-sIgG) ELISA (EuroImmun®, Lübeck, Germany), anti-CHIKV specific-IgM (CHIKV-sIgM) and IgG (CHIKV-sIgG) ELISA (EuroImmun®, Lübeck, Germany), anti-DENV specific-IgG (Panbio Dengue IgG Capture ELISA - panbio diagnostics®, Republic of Korea) and anti-DENV specific-IgG (Panbio Dengue IgM Capture ELISA - panbio diagnostics®, Republic of Korea).

Techniques:

Journal: Antiviral research

Article Title: Serial Real-time RT-PCR and serology measurements substantially improve Zika and Dengue virus infection classification in a co-circulation area.

doi: 10.1016/j.antiviral.2019.104638

Figure Lengend Snippet: Decision Tree results. In the flow diagram each level shows the decision criteria and variable to use to predict the disease group. The colored figures show the criteria diagrammatically. The left figure shows the cut points for the initial cutpoint of low DENV-sIgG SV 28. The purple area shows prediction of household patients with ZIKV-sIgG high and the red area shows the prediction of DENV infected patients. The dots show the true disease status of the people who would fall in the purple and red areas. Red dots are true DENV infected patients, green triangles are true ZIKV infected patients and purple squares are Household subjects. The figure on the right is for the initial cutpoint of high DENV-sIgG SV 28. The green area shows prediction of ZIKV infected patients with ZIKV-sIgM change high and the red area shows the prediction of DENV infected patients.

Article Snippet: Serologies of the three arboviruses were measured using commercial Enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions: anti-ZIKV specific-IgM (ZIKV-sIgM) and IgG (ZIKV-sIgG) ELISA (EuroImmun®, Lübeck, Germany), anti-CHIKV specific-IgM (CHIKV-sIgM) and IgG (CHIKV-sIgG) ELISA (EuroImmun®, Lübeck, Germany), anti-DENV specific-IgG (Panbio Dengue IgG Capture ELISA - panbio diagnostics®, Republic of Korea) and anti-DENV specific-IgG (Panbio Dengue IgM Capture ELISA - panbio diagnostics®, Republic of Korea).

Techniques: Infection

Journal: Antiviral research

Article Title: Serial Real-time RT-PCR and serology measurements substantially improve Zika and Dengue virus infection classification in a co-circulation area.

doi: 10.1016/j.antiviral.2019.104638

Figure Lengend Snippet: Apply decision criteria to undefined fever events patients as an example. These are the results of applying both our decision tree model and the manufacturer recommended cutoffs to those subjects with unknown disease status.

Article Snippet: Serologies of the three arboviruses were measured using commercial Enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions: anti-ZIKV specific-IgM (ZIKV-sIgM) and IgG (ZIKV-sIgG) ELISA (EuroImmun®, Lübeck, Germany), anti-CHIKV specific-IgM (CHIKV-sIgM) and IgG (CHIKV-sIgG) ELISA (EuroImmun®, Lübeck, Germany), anti-DENV specific-IgG (Panbio Dengue IgG Capture ELISA - panbio diagnostics®, Republic of Korea) and anti-DENV specific-IgG (Panbio Dengue IgM Capture ELISA - panbio diagnostics®, Republic of Korea).

Techniques:

Journal: Antiviral research

Article Title: Serial Real-time RT-PCR and serology measurements substantially improve Zika and Dengue virus infection classification in a co-circulation area.

doi: 10.1016/j.antiviral.2019.104638

Figure Lengend Snippet: Spaghetti plots for ZIKV and DENV sIgG and sIgM among the three cohorts: ZIKV-sIgG (row 1), ZIKV-sIgM (row 2), DENV-sIgG (row 3) and DENV-sIgM (row 4) by study visit for each cohort: Definitive Dengue, Definitive Zika and Household. Each colored line represents the trajectory of one subject in the study. The horizontal black line represents the manufacturer-recommended cutoff for defining seropositivity.

Article Snippet: Laboratory procedures Serologies of the three arboviruses were measured using commercial Enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions: anti-ZIKV specific-IgM (ZIKV-sIgM) and IgG (ZIKV-sIgG) ELISA (EuroImmun®, Lübeck, Germany), anti-CHIKV specific-IgM (CHIKV-sIgM) and IgG (CHIKV-sIgG) ELISA (EuroImmun®, Lübeck, Germany), anti-DENV specific-IgG (Panbio Dengue IgG Capture ELISA - panbio diagnostics®, Republic of Korea) and anti-DENV specific-IgG (Panbio Dengue IgM Capture ELISA - panbio diagnostics®, Republic of Korea).

Techniques:

Journal: Antiviral research

Article Title: Serial Real-time RT-PCR and serology measurements substantially improve Zika and Dengue virus infection classification in a co-circulation area.

doi: 10.1016/j.antiviral.2019.104638

Figure Lengend Snippet: Decision Tree results. In the flow diagram each level shows the decision criteria and variable to use to predict the disease group. The colored figures show the criteria diagrammatically. The left figure shows the cut points for the initial cutpoint of low DENV-sIgG SV 28. The purple area shows prediction of household patients with ZIKV-sIgG high and the red area shows the prediction of DENV infected patients. The dots show the true disease status of the people who would fall in the purple and red areas. Red dots are true DENV infected patients, green triangles are true ZIKV infected patients and purple squares are Household subjects. The figure on the right is for the initial cutpoint of high DENV-sIgG SV 28. The green area shows prediction of ZIKV infected patients with ZIKV-sIgM change high and the red area shows the prediction of DENV infected patients.

Article Snippet: Laboratory procedures Serologies of the three arboviruses were measured using commercial Enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions: anti-ZIKV specific-IgM (ZIKV-sIgM) and IgG (ZIKV-sIgG) ELISA (EuroImmun®, Lübeck, Germany), anti-CHIKV specific-IgM (CHIKV-sIgM) and IgG (CHIKV-sIgG) ELISA (EuroImmun®, Lübeck, Germany), anti-DENV specific-IgG (Panbio Dengue IgG Capture ELISA - panbio diagnostics®, Republic of Korea) and anti-DENV specific-IgG (Panbio Dengue IgM Capture ELISA - panbio diagnostics®, Republic of Korea).

Techniques: Infection

Journal: Arteriosclerosis, thrombosis, and vascular biology

Article Title: A novel autoimmune IgM antibody attenuates atherosclerosis in IgM deficient low fat diet-fed, but not Western diet-fed Apoe −/− mice

doi: 10.1161/ATVBAHA.119.312771

Figure Lengend Snippet: (A) IgM levels in serum of Apoe−/− and sIgM−/−Apoe−/− mice at baseline (BL) and after Western diet (WD) feeding. Values = mean ± SEM, n = 10 Apoe−/− BL, 10 sIgM−/− Apoe−/− BL, 7 Apoe−/− BL, 7 sIgM−/−Apoe−/−. (B, C) Sudan IV en face staining (B) and quantification (C) of lesion area in whole aorta of Apoe−/− and sIgM−/− Apoe−/− mice after 12 weeks of WD feeding. Values = mean ± SEM. (D,E) Sudan IV en face staining (D) and quantification (E) of lesion area in the whole aorta of standard laboratory diet-fed, 40 weeks old Apoe−/− and sIgM−/−Apoe−/− mice. Values = mean ± SEM. *P < 0.05 and ***P < 0.001, data analyzed by Student’s t-test. (F) Serum lipid profile analysis in standard laboratory diet-fed, 40 weeks old Apoe−/− and sIgM−/− Apoe−/− mice.

Article Snippet: Apoe −/− mice (The Jackson Laboratory; #002052), ROSA26-STOP Flox eYFP +/+ ;Myh11-CreER T2 ;Apoe −/− (designated as SMC YFP +/+ ApoE −/− ) 9 , 30 , 31 , secretory IgM −/− ( sIgM −/− ) mice were kindly provided by Dr. Peter Lobo (University of Virginia) and crossed to ApoE −/− mice.

Techniques: Western Blot, Staining

Journal: Arteriosclerosis, thrombosis, and vascular biology

Article Title: A novel autoimmune IgM antibody attenuates atherosclerosis in IgM deficient low fat diet-fed, but not Western diet-fed Apoe −/− mice

doi: 10.1161/ATVBAHA.119.312771

Figure Lengend Snippet: (A) Experimental design. sIgM−/− Apoe−/− mice were fed a low fat standard laboratory diet (SD) for 32 weeks. Between 32 – 40 weeks of age, mice were IP injected with 10C12 (n = 11) or IgM control (IgMc, n = 8) twice a week for 8 weeks. Arrows indicate when blood was collected for analyses. (B) Lipid profile and body weight analyses. Values = means ± SEM. (C) Mice treated with the 10C12 antibody and IgMc demonstrated equal IgM levels after eight weeks of injections based on IgM ELISA. NS – non significant by Student t-test. (D) Representative Sudan IV stained en face aorta preparations from sIgM−/− Apoe−/− mice treated with IgMc or 10C12. (E) 10C12 treated mice exhibited reduced Sudan IV positive lesion area as compared to IgM treated control mice. Values = mean ± SEM. *P < 0.05 10C12 versus IgMc by Mann-Whitney test. (F) Quantification of the percentage of lipid accumulation within the tunica media based on the Oil Red O staining of the cross-sections of atherosclerotic lesions within BCA of 10C12 treated mice compared to IgMc treated mice. Values represent mean ± SEM. #P = 0.0582 between treatments by 2-way ANOVA.

Article Snippet: Apoe −/− mice (The Jackson Laboratory; #002052), ROSA26-STOP Flox eYFP +/+ ;Myh11-CreER T2 ;Apoe −/− (designated as SMC YFP +/+ ApoE −/− ) 9 , 30 , 31 , secretory IgM −/− ( sIgM −/− ) mice were kindly provided by Dr. Peter Lobo (University of Virginia) and crossed to ApoE −/− mice.

Techniques: Injection, Control, Enzyme-linked Immunosorbent Assay, Staining, MANN-WHITNEY

Journal: Cancer discovery

Article Title: NUP98-PHF23 is a chromatin modifying oncoprotein that causes a wide array of leukemias sensitive to inhibition of PHD domain histone reader function

doi: 10.1158/2159-8290.CD-13-0419

Figure Lengend Snippet: (A) AML from mouse 820; (i) FACS profiles show Mac1+/Gr1+ and Mac1+/B220dim cells in the bone marrow. The B220+ cells are CD19 and sIgM negative (red arrows) and are therefore not typical B-cells. (ii) H&E and MPO IHC of spleen, thymus and liver showing loss of normal organ architecture and infiltration of MPO+ malignant cells. (iii) May-Grunwald Giemsa (MGG) stained BM myeloblasts.

Article Snippet: Flow cytometry, Immunohistochemistry (IHC) and Immunoblotting Flow assisted cell sorting (FACS) was performed as described elsewhere ( 16 ) with the following conjugated antibodies; Mac1 PE, Gr1 FITC, B220 APC, B220 FITC, CD19 PE, surface IgM PE, CD43 FITC, CD3 PE, CD4 APC, CD8 FITC, CD71 PE, Ter119 FITC, Sca-1 PE, cKit FITC, cKit APC-Cy7, IL7Ra PECy7, CD34 FITC, CD16/32 PE (eBiosciences or BD Biosciences).

Techniques: Staining